[生物活性“肠高血糖素”（胃动素）：胰高血糖素分子C末端延伸的证据]

[Bioactive "enteroglucagon" (oxyntomodulin): evidence for a C-terminal extension of the glucagon molecule].

作者信息

Bataille D, Tatemoto K, Coudray A M, Rosselin G, Mutt V

出版信息

C R Seances Acad Sci III. 1981 Oct 19;293(6):323-8.

Abstract

The bioactive fraction of "enteroglucagon" has been isolated from the porcine jejuno-ileum on the basis of its glucagon-like action in liver. High performance liquid chromatography analysis followed by Dansylation of peptide fragments obtained after enzymatic digestion of both this peptide and glucagon suggests that the bioactive "enteroglucagon" contains the glucagon molecule plus a C-terminal extension under the from of the octapeptide. Lys-Arg-Asn-Lys-Asn-Asn-Ile-Ala-COOH. The presence in the native molecular of a structural difference of modification as compared to glucagon in the 1-11 fragment is possible.

摘要

基于其在肝脏中的胰高血糖素样作用，已从猪空肠回肠中分离出“肠促胰高血糖素”的生物活性部分。对该肽和胰高血糖素进行酶消化后获得的肽片段进行丹磺酰化，然后进行高效液相色谱分析，结果表明，具有生物活性的“肠促胰高血糖素”包含胰高血糖素分子以及一个以八肽形式存在的C端延伸部分，即赖氨酸-精氨酸-天冬酰胺-赖氨酸-天冬酰胺-天冬酰胺-异亮氨酸-丙氨酸-COOH。与胰高血糖素的1-11片段相比，天然分子中可能存在结构修饰差异。