生长培养基对百日咳博德特氏菌与金黄色葡萄球菌之间相互作用的影响。

The influence of growth medium on the interactions between Bordetella pertussis and Staphylococcus aureus.

作者信息

Wyatt T D, Greer A

出版信息

J Med Microbiol. 1976 May;9(2):243-6. doi: 10.1099/00222615-9-2-243.

Abstract

Previous observers showed that many strains of Staphylococcus aureus stimulated the growth of Bordetella pertussis but we have found the reverse: the growth of all available strains of B. pertussis on charcoal-agar medium was inhibited by a standard strain of S. aureus; and 17 of 18 strains of S. aureus (as well as several other organisms) inhibited the growth of a standard strain of B. pertussis. All inhibiting colonies had an unusual brown colouration on the charcoal agar used in the investigation. Both the brown colouration and the inhibitory property were caused by acid production, probably from the starch in the medium. We therefore suggest that media containing starch and blood should not be used in studies of bacterial interference.

摘要

先前的观察表明，许多金黄色葡萄球菌菌株能刺激百日咳博德特氏菌的生长，但我们发现情况相反：金黄色葡萄球菌的一个标准菌株可抑制所有现有百日咳博德特氏菌菌株在活性炭琼脂培养基上的生长；18株金黄色葡萄球菌中的17株（以及其他几种微生物）可抑制百日咳博德特氏菌一个标准菌株的生长。在该研究中使用的活性炭琼脂上，所有具有抑制作用的菌落都呈现出一种异常的褐色。这种褐色和抑制特性都是由产酸引起的，可能是由培养基中的淀粉产酸。因此，我们建议在细菌干扰研究中不应使用含有淀粉和血液的培养基。