Cochran B H, Lillquist J S, Stiles C D
J Cell Physiol. 1981 Dec;109(3):429-38. doi: 10.1002/jcp.1041090308.
Platelet-derived growth factor (PDGF) and platelet-poor plasma, which lacks PDGF, both induce a rapid increase in the rate of total protein synthesis within quiescent, density-arrested Balb/c-3T3 cells. This stimulation of protein synthesis is associated with an increased aggregation of ribosomes into polyribosomes. Nuclear functions are not required for this response, as demonstrated by the observation that this stimulation of protein synthesis occurs in cells pretreated with actinomycin D and in enucleated cells (cytoplasts). The response to PDGF persists even after PDGF has been removed from the culture medium, but in contrast, when plasma is removed from the medium, polysomes disaggregate and protein synthesis declines. PDGF and plasma do not function synergistically to increase protein synthesis, whereas they do to induce optimum DNA synthesis. Thus stimulation of the translational apparatus may be necessary for the mitogenic response of Balb/c-3T3 cells to growth factors, but it is not by itself sufficient.
血小板衍生生长因子（PDGF）和缺乏PDGF的乏血小板血浆，均可诱导静止的、密度抑制的Balb/c-3T3细胞内总蛋白合成速率迅速增加。这种对蛋白质合成的刺激与核糖体聚集成多核糖体的增加有关。如用放线菌素D预处理的细胞和去核细胞（胞质体）中蛋白质合成受到刺激这一观察结果所示，这种反应不需要核功能。即使从培养基中去除PDGF后，对PDGF的反应仍会持续，但相比之下，当从培养基中去除血浆时，多核糖体解聚，蛋白质合成下降。PDGF和血浆在增加蛋白质合成方面不存在协同作用，而在诱导最佳DNA合成方面则存在协同作用。因此，对翻译装置的刺激可能是Balb/c-3T3细胞对生长因子产生有丝分裂反应所必需的，但仅此本身并不足够。
