Emery D L, Morrison W I, Buscher G, Nelson R T
J Immunol. 1982 Jan;128(1):195-200.
The participation of genetically restricted cell-mediated lympholysis (CML) in protective immunity against Theileria parva was examined in cattle inoculated with autologous and allogeneic parasitized lymphoblasts. The majority of cattle given 10(7) infected allogeneic cells were protected against infection with the homologous parasite. Animals inoculated with drug-cured lymphoblasts, normal allogeneic lymph node cells (LNC), or a combination of LNC and parasitized allogeneic cells were fully susceptible. The magnitude of the primary CML responses against autologous infected targets (as opposed to that against the allogeneic lymphoblasts inoculated or 3rd-party targets) more accurately correlated with a decreased severity of infection after challenge. Autologous CML responses appeared earlier as cattle were given increasing numbers of parasitized autologous lymphoblasts, and were higher and more persistent with increasing parasitosis. Between 10(2) and 10(5) autologous infected cells induced immunity to T. parva, whereas at least 10(7) allogeneic lymphoblasts were required for comparable protection. The findings provide additional evidence for a protective role of restricted CML responses against T. parva in vivo. When allogeneic infected cells are used for immunization, viable T. parva need to establish infection in host cells to induce adequate protective immunity.
在接种自体和异体寄生淋巴细胞的牛中，研究了基因限制的细胞介导淋巴细胞溶解（CML）在抵抗小泰氏梨浆虫的保护性免疫中的作用。大多数接种10⁷个感染异体细胞的牛对同源寄生虫感染具有抵抗力。接种经药物治愈的淋巴细胞、正常异体淋巴结细胞（LNC）或LNC与寄生异体细胞组合的动物完全易感。针对自体感染靶标的初次CML反应强度（与针对接种的异体淋巴细胞或第三方靶标的反应强度相反）与攻击后感染严重程度降低更准确相关。随着给牛接种的寄生自体淋巴细胞数量增加，自体CML反应出现得更早，并且随着寄生虫病的增加而更高且更持久。10²至10⁵个自体感染细胞可诱导对小泰氏梨浆虫的免疫，而 comparable protection需要至少10⁷个异体淋巴细胞。这些发现为体内限制的CML反应对小泰氏梨浆虫的保护作用提供了额外证据。当使用异体感染细胞进行免疫时，活的小泰氏梨浆虫需要在宿主细胞中建立感染以诱导足够的保护性免疫。
