Emery D L, Eugui E M, Nelson R T, Tenywa T
Immunology. 1981 Jun;43(2):323-36.
Leucocytes from cattle with lethal or sub-lethal infections with Theileria parva were examined for responses in autologous mixed lymphocyte reactions (MLR) and for specific and non-specific cytotoxicity against cultured cell lines. During lethal infections, lymphoblasts isolated from central lymph from days 8 to 14 stimulated proliferation in autologous lymphatic lymphocytes and peripheral blood leucocytes (PBL), although cytotoxicity was not generated in such cultures. After day 14, non-specific cytotoxicity was exhibited by both lymphocyte populations and was maximal in moribund calves. Non-specific lytic activity was also noted in PBL from recovering calves, but disappeared after the elimination of the parasite. Calves immunized against T. parva by the concurrent administration stabilate and long-acting tetracycline showed macroschizont forms of the parasite in host lymphocytes from days 15 to 18 after infection. During this period, lymph node cells from infected calves stimulated MLR-type responses in autologous PBL. However, PBL from immunized calves lysed directly, autologous infected lymphoblasts in a genetically-restricted fashion from days 14 to 21, although additional cytotoxicity was not generated in the MLR. The results imply that when the parasitosis of T. parva is curtailed, specific cell-mediated responses are mounted against parasite-induced antigens in combination with polymorphic host antigens on the leucocyte membrane. These reactions are probably the major immune responses conferring to recovered cattle, immunity against rechallenge with the homologous isolate of T. parva.
对感染致死或亚致死剂量微小泰勒虫的牛的白细胞进行了检测，以观察其在自体混合淋巴细胞反应（MLR）中的反应，以及对培养细胞系的特异性和非特异性细胞毒性。在致死性感染期间，从第8天到第14天从中央淋巴中分离的淋巴母细胞刺激自体淋巴细胞和外周血白细胞（PBL）增殖，尽管在此类培养物中未产生细胞毒性。14天后，两种淋巴细胞群体均表现出非特异性细胞毒性，在濒死犊牛中达到最大。在康复犊牛的PBL中也观察到非特异性溶解活性，但在寄生虫消除后消失。通过同时接种稳定株和长效四环素免疫的犊牛，在感染后第15天至18天在宿主淋巴细胞中显示出寄生虫的大裂殖体形式。在此期间，感染犊牛的淋巴结细胞刺激自体PBL中的MLR型反应。然而，免疫犊牛的PBL从第14天到21天以基因限制的方式直接裂解自体感染的淋巴母细胞，尽管在MLR中未产生额外的细胞毒性。结果表明，当微小泰勒虫的寄生虫病得到控制时，针对寄生虫诱导的抗原与白细胞膜上的多态性宿主抗原结合，会产生特异性细胞介导的反应。这些反应可能是赋予康复牛对微小泰勒虫同源分离株再次攻击的免疫力的主要免疫反应。
