Woods L F, Wood J M, Gibbs P A
J Gen Microbiol. 1981 Aug;125(2):399-406. doi: 10.1099/00221287-125-2-399.
The phosphoroclastic system was demonstrated in cell-free extracts of clostridium sporogenes by the production of carbon dioxide, acetyl phosphate, ATP and reduced NAD in the presence of pyruvate. The kinetics of acetyl phosphate production and NAD reduction were investigated. The addition of sodium nitrite to a suspension of C. sporogenes in glucose medium resulted in a rapid decrease in intracellular ATP concentration which was accompanied by an accumulation of pyruvate in the medium. This accumulation of pyruvate was caused by inhibition of phosphoroclastic system by nitrate. Nitrite inhibits this system by reaction of nitric oxide, formed from nitrate, with the non-haem iron of pyruvate:ferredoxin oxidoreductase.
在丙酮酸盐存在的情况下，通过二氧化碳、乙酰磷酸、三磷酸腺苷（ATP）和还原型烟酰胺腺嘌呤二核苷酸（NAD）的生成，在生孢梭菌的无细胞提取物中证实了磷酸裂解系统。研究了乙酰磷酸生成和NAD还原的动力学。向葡萄糖培养基中生孢梭菌的悬浮液中添加亚硝酸钠，导致细胞内ATP浓度迅速下降，同时培养基中丙酮酸盐积累。丙酮酸盐的这种积累是由硝酸盐对磷酸裂解系统的抑制引起的。亚硝酸盐通过由硝酸盐形成的一氧化氮与丙酮酸盐：铁氧化还原蛋白氧化还原酶的非血红素铁反应来抑制该系统。
