Conrad J, Zahn R K, Kurelec B, Uhlenbruck G, Müller W E
J Supramol Struct Cell Biochem. 1981;17(1):1-9. doi: 10.1002/jsscb.380170102.
Antibodies were raised against the purified aggregation factor from Geodia cydonium in order to clarify its function during cell aggregation in the homologous and heterologous system. These antibodies inhibited only cell aggregation in the homologous Geodia system and were inactive in the heterologous Tethya lyncurium system. These findings directly indicated that the species-specific reaggregation of sponge cells was initiated by the soluble aggregation factor as already assumed in earlier studies. The amount of neutralizing antibodies was determined by a precipitation reaction with the antigen in capillaries and by microdiffusion. By using the latter technique we got evidence that the Geodia aggregation factor contained a component that was antigenetically related to a galactose-specific lectin present in Geodia cydonium.
为了阐明来自地中海海绵（Geodia cydonium）的纯化聚集因子在同源和异源系统细胞聚集中的功能，制备了针对该因子的抗体。这些抗体仅抑制同源的地中海海绵系统中的细胞聚集，而在异源的林氏浴海绵（Tethya lyncurium）系统中无活性。这些发现直接表明，正如早期研究所设想的那样，海绵细胞的物种特异性重新聚集是由可溶性聚集因子引发的。通过与毛细血管中的抗原进行沉淀反应和微扩散来测定中和抗体的量。通过使用后一种技术，我们获得证据表明，地中海海绵聚集因子含有一种与地中海海绵中存在的半乳糖特异性凝集素在抗原上相关的成分。
