K-562细胞微粒体组分中红细胞聚糖的无细胞生物合成。

Cell-free biosynthesis of erythroglycan in a microsomal fraction from K-562 cells.

作者信息

Russin T Z, Laine R A, Turco S J

出版信息

Biochem J. 1981 Aug 1;197(2):327-32. doi: 10.1042/bj1970327.

DOI:10.1042/bj1970327

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1163131/

Abstract

Particulate membrane preparations from K-562 [human CML (chronic-myelogenous-leukaemia)-derived] cells catalyse the transfer of [3H]galactose from UDP-[3H]-galactose and [3H]N-acetylglucosamine from UDP-[3H]N-acetylglucosamine into an endogenous product that on digestion with Pronase yields long-chain glycopeptides (mol.wt. 7000--10 000) called 'erythroglycan'. Incorporation of either labelled sugar increased up to 60 min of incubation time. The labelled erythroglycan was isolated by chromatography on Sephadex G-50 and characterized by digestion with endo-beta-galactosidase from Escherichia freundii, followed by analysis on Bio-Gel P-2 and paper chromatography. This digestion gave the following four products: (1) a disaccharide with the sequence beta GlcNAc-beta Gal; (2) a trisaccharide with the sequence betaGal-betaGlcNAc-beta Gal; (3) a larger oligosaccharide containing galactose and N-acetylglucosamine; and (4) a putative protein-linkage region.

摘要

来自K-562 [源自人慢性粒细胞白血病（CML）]细胞的微粒体膜制剂催化将UDP-[3H]-半乳糖中的[3H]半乳糖和UDP-[3H] -N-乙酰葡糖胺中的[3H] -N-乙酰葡糖胺转移到一种内源性产物中，该产物经链霉蛋白酶消化后产生称为“红细胞聚糖”的长链糖肽（分子量7000 - 10000）。两种标记糖的掺入在孵育60分钟内均增加。标记的红细胞聚糖通过在Sephadex G-50上进行色谱分离，并通过用弗氏埃希氏菌的内切β-半乳糖苷酶消化，然后在Bio-Gel P-2上进行分析和纸色谱法进行表征。这种消化产生了以下四种产物：（1）具有βGlcNAc-βGal序列的二糖；（2）具有βGal-βGlcNAc-βGal序列的三糖；（3）一种含有半乳糖和N-乙酰葡糖胺的较大寡糖；（4）一个假定的蛋白质连接区域。

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Nature. 1954 Nov 13;174(4437):930-1. doi: 10.1038/174930b0.

2

Structure of an unusual complex-type oligosaccharide isolated from Chinese hamster ovary cells.从中国仓鼠卵巢细胞中分离出的一种异常复合型寡糖的结构

Arch Biochem Biophys. 1980 Feb;199(2):393-9. doi: 10.1016/0003-9861(80)90295-7.

3

Presence of erythroglycan on human K-562 chronic myelogenous leukemia-derived cells.

J Biol Chem. 1980 Apr 25;255(8):3266-9.

4

Erythroglycan biosynthesis in K-562 cells. Inhibition of synthesis by tunicamycin and lack of attachment to the G-protein of vesicular-stomatitis virus.K-562细胞中的红细胞聚糖生物合成。衣霉素对合成的抑制作用以及与水疱性口炎病毒G蛋白的非附着性

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5

Separation and some properties of the major proteins of the human erythrocyte membrane.人红细胞膜主要蛋白质的分离及某些性质

Biochem J. 1972 Sep;129(2):333-47. doi: 10.1042/bj1290333.

6

Deoxyribonucleic acid polymerase III of Escherichia coli. Purification and properties.大肠杆菌的脱氧核糖核酸聚合酶III。纯化及特性

J Biol Chem. 1975 Jan 25;250(2):461-9.

7

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J Biol Chem. 1976 Oct 10;251(19):6108-16.

8

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10

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