Boukhriss-Mghazli S, Mitrovic D
C R Seances Acad Sci III. 1981 Nov 9;293(9):535-8.
In the presence of different types of native collagens, particularly type III, isolated chondrocytes do not attach as usual to the plastic walls of culture flasks, but instead aggregate and form nodules and floating pellicules. Furthermore, articular chondrocytes grow differently in the absence or presence of collagen. In the presence of collagen they grow more rapidly and form nodules and large pellicules. Foetal calf and horse serum and bovine synovial fluid contain dialysable factor(s) that inhibit attachment of the cells to the culture walls, but enhance collagen-induced cell aggregation of the chondrocytes.
在存在不同类型的天然胶原蛋白，尤其是III型胶原蛋白的情况下，分离出的软骨细胞不像往常那样附着在培养瓶的塑料壁上，而是聚集形成结节和漂浮的薄膜。此外，关节软骨细胞在有无胶原蛋白的情况下生长方式不同。在有胶原蛋白存在时，它们生长得更快，并形成结节和大的薄膜。胎牛血清、马血清和牛滑液中含有可透析因子，这些因子可抑制细胞附着于培养壁，但可增强胶原蛋白诱导的软骨细胞聚集。
