通过直接光子吸收法对大鼠股骨进行骨矿物质分析。

Bone mineral analysis of the rat femur by direct photon absorptiometry.

作者信息

Sanchez T V, Myers R C, Bond J T, Mayor G H

出版信息

Calcif Tissue Int. 1981;33(6):587-90. doi: 10.1007/BF02409496.

Abstract

This report describes a method using direct photon absorptiometry (DPA) for determination in vivo of the bone mineral content to bone width ratio (BMC/W) in the rat femur. A significant correlation was noted between measurements by DPA and whole femur dry weight and ash weight. Measurements were reproducible within repeated determinations of both the same and successive days. Soft tissue, rotation of the femur, or slight differences in the longitudinal positioning of the femur did not significantly affect usefulness of the measurements. A significant positive correlation was seen between body weight and the femur BMC/W, indicating that the method accurately reflected expected physiological changes in bone mineral. The method is suitable for the rapid, simple, accurate, and reproducible measurement of bone mineral in the anesthetized rat and is applicable to serial measurements.

摘要

本报告描述了一种使用直接光子吸收法（DPA）在体内测定大鼠股骨骨矿物质含量与骨宽度之比（BMC/W）的方法。DPA测量值与整个股骨干重和灰重之间存在显著相关性。在同一天和连续几天的重复测定中，测量结果具有可重复性。软组织、股骨的旋转或股骨纵向定位的微小差异对测量的有效性没有显著影响。体重与股骨BMC/W之间存在显著正相关，表明该方法准确反映了预期的骨矿物质生理变化。该方法适用于对麻醉大鼠的骨矿物质进行快速、简单、准确和可重复的测量，并且适用于系列测量。