Ben-Jonathan N, Braw R H, Laufer N, Reich R, Bahr J M, Tsafriri A
Endocrinology. 1982 Feb;110(2):457-61. doi: 10.1210/endo-110-2-457.
The purpose of the present study was to measure norepinephrine (NE) in Graafian follicles and correlate changes in its concentration with circulating gonadotropins secreted endogenously or administered exogenously. Graafian follicles were removed from the ovaries of adult cycling rats. The follicles were pooled in groups of 10-13, and NE was determined by high performance liquid chromatography. Follicular NE(picograms per micrograms protein) did not change between 0900 h (3.61 +/- 0.34) and 1300 h (3.12 +/- 0.25) on proestrus, but was reduced significantly to 1.45 +/- 0.16 at 2100 h, which is 4 h after the peak of the gonadotropin surge. There was a further reduction to 0.83 +/- 0.08 in fresh corpora lutea taken on estrus at 0900 h. The decrease in follicular NE was prevented in estrous rats which were either hypophysectomized 24 h previously or treated with sodium pentobarbital at 1330 h on proestrus. To determine which pituitary hormone was responsible for follicular NE depletion, rats were injected at 0900 h on proestrus with LH (5 micrograms), FSH (20 micrograms), LH plus FSH (5 and 20 micrograms, respectively), or PRL (20 micrograms), and follicular NE was determined 4 h later. FSH reduced follicular NE significantly to 1.86 +/- 0.16 compared to both the control (3.12 +/- 0.25) and the PRL-injected group (2.92 +/- 0.32), whereas LH caused a small but nonsignificant decrease (2.49 +/- 0.2). Both LH and FSH doses used resulted in ovulation, as determined by counting tubal ova 12 h after hormonal treatment. We conclude that 1) NE in Graafian follicles is markedly reduced within 4 h after the preovulatory gonadotropin surge in the normal cycling rat; this reduction is prevented when the surge is abolished; 2) the hormone responsible for follicular NE depletion is FSH rather than LH or PRL; and 3) finally, it is suggested that follicular NE may be involved with the formation and/or functioning of the corpus luteum.
本研究的目的是测量格拉夫卵泡中的去甲肾上腺素（NE），并将其浓度变化与内源性分泌或外源性给予的循环促性腺激素相关联。从成年性周期大鼠的卵巢中取出格拉夫卵泡。将卵泡按10 - 13个一组进行汇集，并用高效液相色谱法测定NE。在动情前期，卵泡NE（皮克/微克蛋白）在09:00时（3.61±0.34）至13:00时（3.12±0.25）之间没有变化，但在21:00时显著降低至1.45±0.16，此时是促性腺激素高峰后4小时。在09:00时取自动情期的新鲜黄体中，NE进一步降至0.83±0.08。在动情期大鼠中，若在24小时前进行垂体切除或在动情前期13:30用戊巴比妥钠处理，则可防止卵泡NE的降低。为了确定哪种垂体激素导致卵泡NE耗竭，在动情前期09:00给大鼠注射LH（5微克）、FSH（20微克）、LH加FSH（分别为5和20微克）或PRL（20微克），并在4小时后测定卵泡NE。与对照组（3.12±0.25）和注射PRL组（2.92±0.32）相比，FSH使卵泡NE显著降低至1.86±0.16，而LH引起了小幅度但不显著的降低（2.49±0.2）。通过在激素处理后12小时计数输卵管卵子确定，所使用的LH和FSH剂量均导致排卵。我们得出结论：1）在正常性周期大鼠中，排卵前促性腺激素高峰后4小时内，格拉夫卵泡中的NE显著降低；当高峰被消除时，这种降低被阻止；2）导致卵泡NE耗竭的激素是FSH而非LH或PRL；3）最后，提示卵泡NE可能参与黄体的形成和/或功能。
