Woods D E, Sokol P A, Iglewski B H
Infect Immun. 1982 Feb;35(2):461-4. doi: 10.1128/iai.35.2.461-464.1982.
The iron concentration of the culture medium used to prepare the inocula influenced the pathogenesis of mouse corneal infections by Pseudomonas aeruginosa. When the parental strain PAO1 was cultured in high-iron medium (5 micrograms of Fe per ml), it was less virulent than when it was cultured in low-iron medium (0.05 microgram of Fe per ml). The iron concentration of the growth medium had no effect on the virulence of a P. aeruginosa mutant which was resistant to the iron regulation of toxin A yields (PAO-toxFeR-18). A severely defective iron transport mutant, PAO-toxFeR-10, was avirulent regardless of the iron concentration of the growth medium. These studies indicate that both iron acquisition and iron regulation of toxin production are important factors in the determination of P. aeruginosa virulence.
用于制备接种物的培养基中铁的浓度影响了铜绿假单胞菌对小鼠角膜感染的致病机制。当亲本菌株PAO1在高铁培养基（每毫升含5微克铁）中培养时，其毒力低于在低铁培养基（每毫升含0.05微克铁）中培养时的毒力。生长培养基中铁的浓度对一株对毒素A产量的铁调节具有抗性的铜绿假单胞菌突变体（PAO-toxFeR-18）的毒力没有影响。一个严重缺陷的铁转运突变体PAO-toxFeR-10，无论生长培养基中铁的浓度如何，均无毒性。这些研究表明，铁的获取和毒素产生的铁调节都是决定铜绿假单胞菌毒力的重要因素。
