O'Rourke K I, Satterfield W C
J Am Vet Med Assoc. 1981 Dec 1;179(11):1144-6.
Sera were collected from 103 individuals of 26 species of nondomestic ruminant animals in 2 zoological collections. Eighteen were presumed not to have absorbed colostral proteins (group 1), 32 had consumed colostrum at least 12 hours before testing (group 2), and 53 were healthy adults (group 3). The gamma-globulin concentration, as determined by zone electrophoresis, was less than or equal to 0.4 g/dl in 16 of 18 samples from the group 1 animals, and greater than 0.4 g/dl in 31 of 32 group 2 animals. The glutaraldehyde coagulation test was performed on each serum sample. The results were negative in 15 of 17 samples, with gamma-globulin concentration less than or equal to 0.4 g/dl, and positive in 85 of 86 samples, with gamma-globulin concentration greater than 0.4 g/dl. The glutaraldehyde coagulation test appeared to be a practical field test for determining failure of passive transfer of maternal antibody in newborn nondomestic ruminant animals in zoological collections.
从两个动物园收集的26种非家养反刍动物的103只个体中采集血清。18只被认为未吸收初乳蛋白（第1组），32只在检测前至少12小时已摄入初乳（第2组），53只为健康成年动物（第3组）。通过区带电泳测定，第1组动物的18份样本中有16份的γ-球蛋白浓度小于或等于0.4 g/dl，第2组动物的32份样本中有31份的γ-球蛋白浓度大于0.4 g/dl。对每份血清样本进行戊二醛凝固试验。γ-球蛋白浓度小于或等于0.4 g/dl的17份样本中有15份结果为阴性，γ-球蛋白浓度大于0.4 g/dl的86份样本中有85份结果为阳性。戊二醛凝固试验似乎是一种用于确定动物园新生非家养反刍动物母体抗体被动转移失败的实用现场试验。
