Hays R M, Bourguet J, Satir B H, Franki N, Rapoport J
J Cell Biol. 1982 Jan;92(1):237-41. doi: 10.1083/jcb.92.1.237.
Aggregates of intramembrane particles appear in the luminal membranes of renal collecting duct and amphibian bladder cells after stimulation by antidiuretic hormone (ADH). We undertook this freeze-fracture study to determine whether particle aggregates, once in place, remain in the luminal membrane of the amphibian bladder after the membrane is physically separated from the rest of the cell. We found that the aggregates do remain in high yield in isolated membranes stabilized with a bifunctional imidoester (DTBP) followed by fixation with glutaraldehyde, or unfixed but stabilized with DTBP. These findings support the view that the particles are intrinsic membrane components and that their organization in the form of aggregates does not depend on the presence of the intact cell. In addition, the availability of isolated membranes containing particle aggregates provides a starting point for the isolation of the water-conducting proteins.
抗利尿激素（ADH）刺激后，肾集合管和两栖类膀胱细胞的管腔膜中会出现膜内颗粒聚集体。我们进行了这项冷冻断裂研究，以确定颗粒聚集体一旦形成，在膜与细胞其余部分物理分离后，是否仍保留在两栖类膀胱的管腔膜中。我们发现，在用双功能亚胺酯（DTBP）稳定后再用戊二醛固定的分离膜中，或未固定但用DTBP稳定的分离膜中，聚集体仍能高产率地保留下来。这些发现支持了这样一种观点，即这些颗粒是内在膜成分，并且它们以聚集体形式的组织不依赖于完整细胞的存在。此外，含有颗粒聚集体的分离膜的可得性为分离水传导蛋白提供了一个起点。
