罂粟碱对从大鼠子宫平滑肌分离出的线粒体组分摄取钙离子的影响。

Effect of papaverine on Ca2+ uptake by a mitochondrial fraction isolated from rat uterine smooth muscle.

作者信息

Sakai K, Takayanagi I, Uchida M, Takagi K

出版信息

Eur J Pharmacol. 1978 Jul 15;50(2):131-6. doi: 10.1016/0014-2999(78)90008-0.

Abstract

A mitochondrial fraction was isolated from rat uterine smooth muscle. The effect of papaverine (3 X 10(-6), 10(-5), 3 X 10(-5), 10(-4) M) on Ca2+ accumulation by the fraction was studied. The effect of papaverine was changed by using different substrates for Ca2+ accumulation. Papaverine inhibited the Ca2+ accumulation supported by glutamate plus ATP concentration-dependently. The Ca2+ accumulation supported by ATP alone was also inhibited. On the other hand, the Ca2+ accumulation supported by succinate and ATP was increased by papaverine. The effect was not concentration-dependent; papaverine (3 X 10(-5) M) was the most effective concentration for the effect with a shorter incubation (2 min).

摘要

从大鼠子宫平滑肌中分离出线粒体部分。研究了罂粟碱（3×10⁻⁶、10⁻⁵、3×10⁻⁵、10⁻⁴M）对该部分线粒体钙积累的影响。通过使用不同的钙积累底物，罂粟碱的作用发生了变化。罂粟碱浓度依赖性地抑制了由谷氨酸加ATP支持的钙积累。仅由ATP支持的钙积累也受到抑制。另一方面，由琥珀酸和ATP支持的钙积累则因罂粟碱而增加。该作用不依赖于浓度；罂粟碱（3×10⁻⁵M）在较短孵育时间（2分钟）时是产生该作用的最有效浓度。