Nemes Z, Thomázy V, Szeifert G
J Immunol Methods. 1982;49(1):53-64. doi: 10.1016/0022-1759(82)90365-9.
The application of freeze-dried paraffin sections for immunohistology eliminates many of the problems associated with the cryostat technique: the sections are thin sufficiently, the cell morphology is improved, there are no diffusion artifacts and the intensity of immunohistological reactions is superior to that obtained on cryostat sections. The preservation of antogenicity in freeze-dried paraffin sections is sufficient for the demonstration of light chain monotypia in lymphomas of B-cell origin. Indirect immunofluorescence is the method of choice for demonstrating surface Ig in freeze-dried paraffin sections.
切片足够薄，细胞形态得到改善，不存在扩散假象，并且免疫组织学反应的强度优于低温恒冷切片。冻干石蜡切片中抗原性的保存足以用于显示B细胞起源淋巴瘤中的轻链单型性。间接免疫荧光是在冻干石蜡切片中显示表面免疫球蛋白的首选方法。
