Okada M, Yoshimura N, Kaieda T, Yamamura Y, Kishimoto T
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7717-21. doi: 10.1073/pnas.78.12.7717.
Hybridization of human T cells with an azaguanine-resistant human T cell line gave rise to T hybrid cell lines secreting several immunoregulatory molecules. Analyses of karyotypes, HLA phenotypes, and other surface phenotypes, such as T-cell-specific antigens or receptors for sheep erythrocytes and the patterns of mitogen responsiveness confirmed that the hypoxanthine/aminopterin/thymidine-resistant cell lines were human T-T hybridomas. One of the established hybrid clones (24-A) secreted human interleukin-2 (IL-2). The culture supernatants induced the proliferation of concanavalin A-stimulated murine T cells and supported the proliferation of an IL 2-dependent human cytotoxic T-cell line. In a clone (38-B) that did not show any IL-2 activity in culture supernatants, the addition of macrophages induced IL-2 production in the presence of phytohemagglutinin, suggesting that interleukin-1 induced IL-2 production in T hybrid cells. Hybrid cells secreting killer helper factor were also established. The culture supernatants from this clone, 55-A, helped the induction of cytotoxic T cells against UV-treated human B-blastoid cells but did not show any IL-2 activity.
人T细胞与抗氮杂鸟嘌呤的人T细胞系杂交，产生了分泌多种免疫调节分子的T杂交细胞系。对核型、HLA表型以及其他表面表型（如T细胞特异性抗原或绵羊红细胞受体）和丝裂原反应模式的分析证实，次黄嘌呤/氨基蝶呤/胸腺嘧啶核苷抗性细胞系是人T-T杂交瘤。已建立的杂交克隆之一（24-A）分泌人白细胞介素-2（IL-2）。培养上清液诱导刀豆球蛋白A刺激的小鼠T细胞增殖，并支持IL-2依赖性人细胞毒性T细胞系的增殖。在培养上清液中未显示任何IL-2活性的克隆（38-B）中，添加巨噬细胞可在存在植物血凝素的情况下诱导IL-2产生，这表明白细胞介素-1在T杂交细胞中诱导IL-2产生。还建立了分泌杀伤辅助因子的杂交细胞。来自该克隆（55-A）的培养上清液有助于诱导针对紫外线处理的人B淋巴母细胞样细胞的细胞毒性T细胞，但未显示任何IL-2活性。
