兔肾皮质微粒体将花生四烯酸代谢为三羟基二十碳三烯酸。
Rabbit renal cortical microsomes metabolize arachidonic acid to trihydroxyeicosatrienoic acids.
作者信息
Oliw E H, Oates J A
出版信息
Prostaglandins. 1981 Dec;22(6):863-71. doi: 10.1016/0090-6980(81)90017-4.
(1-14C) Eicosatetraenoic (Arachidonic) acid was incubated with microsomes from rabbit renal cortex and NADPH (1 mM) for 15 min at 37 degrees C. The products were extracted and purified by high pressure liquid chromatography. Some of the most polar metabolites were identified by gas chromatography mass spectrometry. They were 11,12,19- and 11,12,20-trihydroxy-5,8,14-eicosatrienoic acid, 14,15,19- and 14,15,20-trihydroxy-5,8,11-eicosatrienoic acid, and 11,12-dihydroxy-19-oxo-5,8,14-eicosatrienoic acid. These products were likely formed by omega-and ( omega-1)-hydroxylation of 11,12-dihydroxy-5,8,14-eicosatrienoic acid and 14,15-dihydroxy-5,8,11-eicosatrienoic acid, two recently identified metabolites of arachidonic acid in fortified rabbit kidney microsomes.
将（1-14C）二十碳四烯酸（花生四烯酸）与兔肾皮质微粒体及1 mM烟酰胺腺嘌呤二核苷酸磷酸（NADPH）在37℃下孵育15分钟。产物通过高压液相色谱法进行提取和纯化。部分极性最强的代谢产物通过气相色谱 - 质谱联用仪进行鉴定。它们分别是11,12,19 - 三羟基 - 5,8,14 - 二十碳三烯酸、11,12,20 - 三羟基 - 5,8,14 - 二十碳三烯酸、14,15,19 - 三羟基 - 5,8,11 - 二十碳三烯酸、14,15,20 - 三羟基 - 5,8,11 - 二十碳三烯酸以及11,12 - 二羟基 - 19 - 氧代 - 5,8,14 - 二十碳三烯酸。这些产物可能是由11,12 - 二羟基 - 5,8,14 - 二十碳三烯酸和14,15 - 二羟基 - 5,8,11 - 二十碳三烯酸经ω - 和（ω - 1） - 羟基化形成的，这两种是最近在强化兔肾微粒体中鉴定出的花生四烯酸代谢产物。
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