Gräfe U, Roth M, Christner A, Bormann E J
Z Allg Mikrobiol. 1981;21(9):633-42. doi: 10.1002/jobm.3630210902.
Five non-streptomycin-producing mutants of an industrial strain of Streptomyces griseus lacking aerial mycelium formation were compared with their genetic ancestor and another producing mutant with regard to the NAD(P)-glycohydrolase activity during cultivation on different media. By contrast to producing strains, all the Str- Amy- mutants displayed much lower mycelial and extracellular levels of enzyme, thus confirming earlier contentions concerning its involvement in control by phospho-adenosinediphospho-ribose of the intermediary metabolism at the sites of the citric acid cycle and the catabolism of the carbohydrates during distinct stages of cell differentiation. On the other hand, in the producing strain the biosynthesis of NAD(P)-glycohydrolase was demonstrated to depend on the regime of fermentation. Repeated stages of submerged cultivation in stirred fermentors resulted in suppression of enzyme formation without concomitant change of the mycelial capacity to produce streptomycin. This suggests that there is no direct involvement of NAD(P)-glycohydrolase in the control of antibiotic biosynthesis.
将一株不产链霉素且缺乏气生菌丝体形成的灰色链霉菌工业菌株的五个非链霉素产生突变体，与其遗传祖先以及另一个产生突变体，就其在不同培养基上培养期间的NAD(P)-糖水解酶活性进行了比较。与产生菌株相比，所有Str-Amy-突变体的菌丝体和细胞外酶水平都低得多，从而证实了早期有关其在柠檬酸循环位点和细胞分化不同阶段碳水化合物分解代谢中通过磷酸腺苷二磷酸核糖参与中间代谢控制的论点。另一方面，在产生菌株中，NAD(P)-糖水解酶的生物合成被证明取决于发酵方式。在搅拌发酵罐中反复进行深层培养阶段会导致酶形成受到抑制，而菌丝体产生链霉素的能力没有随之改变。这表明NAD(P)-糖水解酶不直接参与抗生素生物合成的控制。
