Troianovskiĭ S M
Biull Eksp Biol Med. 1982 Jan;93(1):81-4.
Unlike nontumorigenic IAR2 cells, no surface fibronectin was detected in tumorigenic epithelial liver cells, IAR2-31 and IAR6-IRT7A. The adsorption of exogenous fibronectin purified from rat plasma, onto the substratum before seeding cells led to varying results in different cell lines. IAR2 showed no morphological changes or changes in the actin cytoskeleton, but acquired a network of radially oriented fibronectin fibrils. Under the effect of fibronectin single IAR2-31 cells returned to normal in terms of both the morphology and actin cytoskeleton, as well as acquired radially oriented fibronectin fibrils. At the same time IAR2-31 cells in the sheet were characterized by a circular actin bundle which made them essentially differ from IAR2 cells. IAR6-IRT7A cells were found to lose the ability to return to normal on fibronectin-coated substrates.
与非致瘤性IAR2细胞不同，在致瘤性上皮肝细胞IAR2 - 31和IAR6 - IRT7A中未检测到表面纤连蛋白。在接种细胞前将从大鼠血浆中纯化的外源性纤连蛋白吸附到基质上，在不同细胞系中产生了不同的结果。IAR2没有形态变化或肌动蛋白细胞骨架的改变，但获得了呈放射状排列的纤连蛋白纤维网络。在纤连蛋白的作用下，单个IAR2 - 31细胞在形态和肌动蛋白细胞骨架方面恢复正常，同时也获得了呈放射状排列的纤连蛋白纤维。与此同时，单层培养的IAR2 - 31细胞具有圆形肌动蛋白束，这使得它们与IAR2细胞有本质区别。发现IAR6 - IRT7A细胞在纤连蛋白包被的底物上失去了恢复正常的能力。
