新生仓鼠磨牙的牙齿发育：釉原蛋白、釉蛋白和可溶性牙本质蛋白的提取与表征

Neonatal hamster molar tooth development: extraction and characterization of amelogenins, enamelins, and soluble dentin proteins.

作者信息

Lyaruu D M, Belcourt A, Fincham A G, Termine J D

出版信息

Calcif Tissue Int. 1982 Jan;34(1):86-96. doi: 10.1007/BF02411214.

Abstract

Amelogenins, enamelins, and soluble dentin proteins were sequentially separated under dissociative conditions from morphologically characterized molar tooth germs of 4-, 6-, and 7-day-old hamsters. Polyacrylamide gel electrophoretic, gel filtration chromatographic, and amino acid compositional data of neonatal hamster amelogenin extracts were in general agreement with those obtained from fetal bovine enamel under similar extraction conditions. As development progressed (e.g., 4 vs. 7 days of life), changes in amelogenin proteins were manifested by altered values of all biochemical parameters measured. A high molecular weight (approximately 160,000-200,000 daltons) Stains-All-positive protein band was observed for all hamster enamelin extracts on SDS gels. Amino acid compositional data from this "enamel crystal protein," partially purified by dissociative gel-filtration chromatography, are presented. The hamster dentin phosphoprotein was partially purified by ion-exchange chromatography in 7M urea. The molecular weight (75,000-80,000 daltons) and amino acid composition of this protein were similar to those of rat incisor dentin phosphoprotein, but different from those of the fetal bovine phosphoprotein.

摘要

在解离条件下，从4日龄、6日龄和7日龄仓鼠形态特征明确的磨牙牙胚中依次分离出釉原蛋白、釉蛋白和可溶性牙本质蛋白。新生仓鼠釉原蛋白提取物的聚丙烯酰胺凝胶电泳、凝胶过滤色谱和氨基酸组成数据与在类似提取条件下从胎牛牙釉质中获得的数据总体一致。随着发育进程（例如，出生4天与7天相比），釉原蛋白的变化表现为所测所有生化参数值的改变。在SDS凝胶上，所有仓鼠釉蛋白提取物均观察到一条高分子量（约160,000 - 200,000道尔顿）的耐尔蓝染色阳性蛋白带。本文给出了通过解离凝胶过滤色谱部分纯化的这种“釉质晶体蛋白”的氨基酸组成数据。仓鼠牙本质磷蛋白通过在7M尿素中进行离子交换色谱法进行了部分纯化。该蛋白的分子量（75,000 - 80,000道尔顿）和氨基酸组成与大鼠切牙牙本质磷蛋白相似，但与胎牛磷蛋白不同。