Peterkofsky B, Prather W
Cancer Res. 1982 May;42(5):1809-16.
The relationship between transformation, lactate production, and glucose transport was examined in a series of ten cell lines consisting of subclones of BALB 3T3 A31 cells and viral and chemical transformants of either the subclones or the original A31 line. Comparisons were made over a relatively narrow range of cell densities to minimize changes in the biochemical parameters during growth. A nitroquinoline oxide (NQT-3T3-714) and a temperature-sensitive Kirsten sarcoma virus (tsKi-3T3-714) transformant of subclone 714 exhibited transformed phenotypes with respect to morphology and growth properties, but their rates of lactate production and 2-[3H]deoxy-D-glucose (deoxyglucose) uptake were similar to those of the parent cells. 2- to 5-fold in these transformants, showing that there was no defect in the enzymes of this pathway. At a temperature nonpermissive for transformation of tsKi-3T3-714, lactate production by this line did not decrease relative to the rate of the parent cells. Another transformant, Ki-3T3-234, had a glycolytic rate which was 4 to 5 times greater than that of the low lactate producers while other transformants exhibited intermediate rates, and the rate of a third nontransformed 3T3 A31 subclone, K-1-1, was comparable to the rate of Ki-3T3-234. The rates of [3H]deoxyglucose uptake by this series of cells were closely proportional to their glycolytic rates rather than to their state of transformation. Increasing glycolysis by oligomycin or dinitrophenol treatment, however, did not cause a concomitant increase in sugar uptake. Neither glycolysis nor deoxyglucose uptake in the high-lactate producer (Ki-3T3-234) was inhibited by ouabain, suggesting that Na+-K+-adenosinetriphosphatase is not a regulatory of these functions in 3T3 cells. In 3T3-derived cells, it appears that the rates of glycolysis and glucose uptake may be regulated in tandem under some conditions and that neither process is an obligatory consequence of neoplastic transformation
在由BALB 3T3 A31细胞亚克隆以及这些亚克隆或原始A31细胞系的病毒和化学转化体组成的一系列十个细胞系中，研究了转化、乳酸产生和葡萄糖转运之间的关系。在相对较窄的细胞密度范围内进行比较，以尽量减少生长过程中生化参数的变化。亚克隆714的硝基喹啉氧化物（NQT - 3T3 - 714）和温度敏感型 Kirsten 肉瘤病毒（tsKi - 3T3 - 714）转化体在形态和生长特性方面表现出转化表型，但其乳酸产生率和2 - [³H]脱氧 - D - 葡萄糖（脱氧葡萄糖）摄取率与亲本细胞相似。这些转化体中的乳酸产生率和脱氧葡萄糖摄取率比亲本细胞高2至5倍，表明该途径的酶没有缺陷。在tsKi - 3T3 - 714转化不允许的温度下，该细胞系的乳酸产生相对于亲本细胞的速率并未降低。另一个转化体Ki - 3T3 - 234的糖酵解速率比低乳酸产生者高4至5倍，而其他转化体表现出中间速率，第三个未转化的3T3 A31亚克隆K - 1 - 1的速率与Ki - 3T3 - 234的速率相当。这一系列细胞的[³H]脱氧葡萄糖摄取率与其糖酵解速率密切相关，而不是与其转化状态相关。然而，用寡霉素或二硝基苯酚处理增加糖酵解并不会导致糖摄取相应增加。高乳酸产生者（Ki - 3T3 - 234）中的糖酵解和脱氧葡萄糖摄取均不受哇巴因抑制，这表明Na⁺ - K⁺ - 三磷酸腺苷酶不是3T3细胞中这些功能的调节因子。在源自3T3的细胞中，似乎在某些条件下糖酵解速率和葡萄糖摄取速率可能协同调节，并且这两个过程都不是肿瘤转化的必然结果
