Michaelsen T E, Lea T
Immunology. 1982 Apr;45(4):751-9.
Antisera against a human VH fragment obtained from an IgG3, VH II, kappa protein (KUP) were raised in rabbits, sheep and chicken. The three types of anti-VH antisera reacted equally well with both intact immunoglobulin molecules and isolated heavy chains. The antisera did not detect any free heavy chain specific antigens by sensitive enzyme-linked immunosorbent assay (ELISA) tests although they reacted with some antigens which were more or less hidden on intact immunoglobulin molecules but well expressed on isolated heavy chains. The antisera reacted with more than 90% of IgG, IgA and IgM present in normal pooled serum. Experiments with T cells from normal peripheral blood indicated that the sheep and rabbit anti-VH antisera reacted with a 70,000 mol.wt T-cell surface antigen.
用从一种IgG3、VH II、κ蛋白（KUP）获得的人VH片段制备的抗血清在兔、绵羊和鸡体内产生。这三种抗VH抗血清与完整的免疫球蛋白分子和分离的重链反应同样良好。尽管这些抗血清与一些在完整免疫球蛋白分子上或多或少隐藏但在分离的重链上良好表达的抗原发生反应，但通过灵敏的酶联免疫吸附测定（ELISA）试验，它们未检测到任何游离重链特异性抗原。这些抗血清与正常混合血清中存在的90%以上的IgG、IgA和IgM发生反应。对来自正常外周血的T细胞进行的实验表明，绵羊和兔抗VH抗血清与一种分子量为70,000的T细胞表面抗原发生反应。
