Moore M A
J Cell Physiol Suppl. 1982;1:53-64. doi: 10.1002/jcp.1041130411.
The murine myelomonocytic leukemia WEHI-3B exists as differentiation-inducible (D+) and noninducible (D-) cell lines. Both lines produce a CSF species that stimulates exclusively the formation of neutrophil granulocyte colonies. This G-CSF copurifies with a mast cell growth factor but can be separated from M- and GM-CSF. NZB bone marrow is unresponsive to G-CSF stimulation. WEHI-3B D+ cells can induced to terminal granulocyte differentiation by a factor present in murine and human postendotoxin serum that is different from G-CSF present in WEHI-3B D+ or D- CM since the latter has little or no leukemia differentiation-inducing activity. Endotoxin treatment of C. parvum primed mice leads to simultaneous induction of serum activities with selective action on myeloid leukemic cells, a serum differentiation inducing activity and a leukemic colony inhibitory activity. These factors act synergistically to block leukemic stem cell self-renewal. The results suggest that a variety of inducible factors may have potent and selective antileukemic activity.
小鼠骨髓单核细胞白血病WEHI-3B以可诱导分化（D+）和不可诱导分化（D-）细胞系的形式存在。这两种细胞系都会产生一种集落刺激因子，该因子仅刺激中性粒细胞集落的形成。这种粒细胞集落刺激因子（G-CSF）与肥大细胞生长因子共同纯化，但可与巨噬细胞集落刺激因子（M-CSF）和粒细胞巨噬细胞集落刺激因子（GM-CSF）分离。新西兰黑鼠（NZB）骨髓对G-CSF刺激无反应。WEHI-3B D+细胞可被鼠和人内毒素血症后的血清中存在的一种因子诱导至终末粒细胞分化，该因子不同于WEHI-3B D+或D-条件培养基（CM）中存在的G-CSF，因为后者几乎没有或没有白血病分化诱导活性。用微小隐孢子虫（C. parvum）预处理的小鼠进行内毒素治疗，可同时诱导血清活性，这些活性对髓系白血病细胞具有选择性作用，即血清分化诱导活性和白血病集落抑制活性。这些因子协同作用以阻断白血病干细胞的自我更新。结果表明，多种诱导因子可能具有强大的选择性抗白血病活性。
