Ager A, Gordon J L, Moncada S, Pearson J D, Salmon J A, Trevethick M A
J Cell Physiol. 1982 Jan;110(1):9-16. doi: 10.1002/jcp.1041100103.
Freshly isolated neonatal porcine aortic tissue (smooth muscle with or without endothelium present) produced approximately 30 ng/mg wet tissue of 6-oxo-prostaglandin F1 alpha (the stable hydrolysis product from prostacyclin) and approximately 15 ng/mg of prostaglandin E2, as measured by radioimmunoassay after 24 h incubation in culture medium. Primary cultures of porcine endothelial and smooth muscle cells (isolated by enzymic digestion of aortic tissue) exhibited the same pattern of prostaglandin production, but absolute values were greater than for fresh tissue, particularly in the case of endothelium. Subcultures of endothelium produced smaller amounts of prostaglandins, although the pattern remained similar. In contrast, subcultures of smooth muscle cells produced a greater total amount of prostaglandins than did primary cultures, and the main product was prostaglandin E2. Experiments with [14C] prostaglandin H2 or [14C]arachidonic acid confirmed that aortic tissue, cultured endothelium, and primary cultures or aortic smooth muscle cells synthesized prostacyclin, and demonstrated that subcultured smooth muscle cells enzymically isomerised prostaglandin H2 to prostaglandin E2. Kinetic studies showed that prostaglandin production by cultured vascular cells was transiently increased by subculture or changing the growth medium, and that production per cell declined with increasing cell density. The change in pattern of prostaglandin production during culture was shown to be due to a rapid decline in the rate of prostacyclin production (which apparently began immediately after tissue isolation), together with a more gradual rise in prostaglandin E2 production. These results indicate that the amounts and ratios of prostaglandins produced by vascular endothelial and smooth muscle cells are greatly affected by the conditions used to isolate and culture the cells; vascular cells in vivo may similarly alter their pattern of prostaglandin production in response to local changes in their environment.
新鲜分离的新生猪主动脉组织（存在或不存在内皮的平滑肌）在培养基中培养24小时后，通过放射免疫测定法测得，产生约30 ng/mg湿组织的6-氧代-前列腺素F1α（前列环素的稳定水解产物）和约15 ng/mg的前列腺素E2。猪内皮细胞和平滑肌细胞的原代培养物（通过主动脉组织的酶消化分离）呈现出相同的前列腺素产生模式，但绝对值高于新鲜组织，尤其是内皮细胞的情况。内皮细胞的传代培养产生的前列腺素量较少，尽管模式仍然相似。相比之下，平滑肌细胞的传代培养产生的前列腺素总量比原代培养物多，主要产物是前列腺素E2。用[14C]前列腺素H2或[14C]花生四烯酸进行的实验证实，主动脉组织、培养的内皮细胞以及原代培养物或主动脉平滑肌细胞合成前列环素，并表明传代培养的平滑肌细胞将前列腺素H2酶促异构化为前列腺素E2。动力学研究表明，培养的血管细胞产生的前列腺素会因传代培养或更换生长培养基而短暂增加，并且每个细胞的产量会随着细胞密度的增加而下降。培养过程中前列腺素产生模式的变化表明，这是由于前列环素产生速率迅速下降（显然在组织分离后立即开始），同时前列腺素E2的产生逐渐增加。这些结果表明，血管内皮细胞和平滑肌细胞产生的前列腺素的量和比例受到分离和培养细胞所用条件的极大影响；体内的血管细胞可能会类似地根据其局部环境的变化改变其前列腺素产生模式。
