Parnham M J, Bittner C, Winkelmann J
Agents Actions. 1981 Dec;11(6-7):617-9. doi: 10.1007/BF01978765.
We are currently characterizing different macrophages sub-populations according to their release of oxygen free radicals (measured as chemiluminescence) under various stimuli and their responses to drugs and mediators. Mouse macrophages were obtained by peritoneal or lung lavage and stimulated in vitro with opsonized zymosan (OpZ) or the calcium ionophore A23187. Chemiluminescence was measured in a luminometer and responses obtained after 5 min expressed as percentages of standard responses to the stimuli. Almost identical dose-response curves to OpZ were obtained with peritoneal and alveolar macrophages, while the dose-response curve of peritoneal macrophages to A23187 was much shallower. The response to A23187 was generally much weaker than that to OpZ and in both types of macrophages PGE2 was a more effective inhibitor of OpZ-induced chemiluminescence than of that induced by A23187. The response to OpZ in peritoneal macrophages was inhibited in a dose-related manner by both PGE2 and superoxide dismutase, but not by indomethacin, d-penicillamine, piroxicam or phenylbutazone. OpZ but not A23187, is a suitable stimulus for pharmacological studies on macrophage chemiluminescence.
我们目前正在根据不同巨噬细胞亚群在各种刺激下释放氧自由基（以化学发光法测定）的情况以及它们对药物和介质的反应来对其进行表征。通过腹腔或肺灌洗获取小鼠巨噬细胞，并在体外用人源化酵母聚糖（OpZ）或钙离子载体A23187进行刺激。在发光计中测量化学发光，并将5分钟后获得的反应表示为对刺激的标准反应的百分比。腹腔巨噬细胞和肺泡巨噬细胞对OpZ的剂量反应曲线几乎相同，而腹腔巨噬细胞对A23187的剂量反应曲线则平缓得多。对A23187的反应通常比对OpZ的反应弱得多，并且在这两种类型的巨噬细胞中，前列腺素E2（PGE2）对OpZ诱导的化学发光的抑制作用比对A23187诱导的化学发光的抑制作用更有效。PGE2和超氧化物歧化酶均以剂量相关的方式抑制腹腔巨噬细胞对OpZ的反应，但吲哚美辛、d-青霉胺、吡罗昔康或苯基丁氮酮则无此作用。OpZ而非A23187是用于巨噬细胞化学发光药理学研究的合适刺激物。
