亲和层析以及将乙酰胆碱受体附着于尼龙管上进行免疫吸附。
Affinity chromatography and immunosorption with acetylcholine receptor attached to nylon tubes.
作者信息
Yang B H, Sundaram P V, Maelicke A
出版信息
Biochem J. 1981 Nov 1;199(2):317-22. doi: 10.1042/bj1990317.
Abstract
Nylon-linked proteins were used for affinity trapping and chromatography. As representative examples purified acetylcholine receptor, alpha-cobratoxin and bovine serum albumin were coupled to the activated matrix to serve as biospecific ligands. In particular, acetylcholine receptor was coupled without significant loss of biochemical properties. The resulting affinity tubes bind receptor-specific ligands including immunoglobulins and thus can be used for affinity-chromatographic purposes and immunoassays.
摘要
尼龙连接的蛋白质用于亲和捕获和色谱分析。作为代表性实例，纯化的乙酰胆碱受体、α-银环蛇毒素和牛血清白蛋白与活化基质偶联，用作生物特异性配体。特别是，乙酰胆碱受体在偶联后其生化特性没有明显损失。所得的亲和管可结合包括免疫球蛋白在内的受体特异性配体，因此可用于亲和色谱分析和免疫测定。
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2
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FEBS Lett. 1980 Oct 20;120(1):145-8. doi: 10.1016/0014-5793(80)81066-0.
3
Translational diffusion coefficient and molecular weight of the acetylcholine receptor from Torpedo marmorata.斑纹电鳐乙酰胆碱受体的平移扩散系数和分子量
FEBS Lett. 1980 May 5;113(2):312-4. doi: 10.1016/0014-5793(80)80616-8.
4
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Proc Natl Acad Sci U S A. 1980 May;77(5):2400-4. doi: 10.1073/pnas.77.5.2400.
5
6
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Proc Natl Acad Sci U S A. 1980 Feb;77(2):755-9. doi: 10.1073/pnas.77.2.755.
7
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J Biol Chem. 1973 Oct 10;248(19):6841-53.
8
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J Biol Chem. 1977 Jul 25;252(14):4811-30.
9
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J Immunol. 1977 Jan;118(1):17-20.
10
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