[5日龄、12日龄和21日龄大鼠中各种氨基酸血脑屏障的比较]

[Comparison of blood-brain barrier of various amino acids in 5, 12 and 21 day old rats].

作者信息

Lefauconnier J M, Trouvé R

出版信息

C R Seances Acad Sci III. 1981 Dec 21;293(15):821-4.

Abstract

The bolus injection technique described by Oldendorf has been modified in order to study the blood brain transport of amino acids in immature Rats. The brain uptake indices (BUI) of lysine, leucine, alanine, proline, glucose and glutamic acid have been compared at tracer dose and at 10 mM concentration in 5, 12 and 21 day-old Rats. At every time-period, the BUIs of proline, glycine and glutamic acid were not decreased by the 10 mM concentration and were similar to that of sucrose. Between day 5 to 21, the saturable fraction of the BUI of lysine decreased by 50% that of leucine increased by about 50% and that of alanine which represented only a small fraction of the total uptake decreased with time; the unsaturable fraction of the BUIs of these 3 amino acids was higher than the BUI of sucrose and declined progressively.

摘要

为了研究未成熟大鼠中氨基酸的血脑转运，对奥尔德恩多夫描述的团注注射技术进行了改进。在5日龄、12日龄和21日龄的大鼠中，比较了赖氨酸、亮氨酸、丙氨酸、脯氨酸、葡萄糖和谷氨酸在示踪剂量和10 mM浓度下的脑摄取指数（BUI）。在每个时间段，脯氨酸、甘氨酸和谷氨酸的BUI在10 mM浓度下并未降低，且与蔗糖的BUI相似。在第5天至第21天之间，赖氨酸BUI的可饱和部分下降了50%，亮氨酸的增加了约50%，而仅占总摄取量一小部分的丙氨酸的BUI随时间下降；这三种氨基酸BUI的不饱和部分高于蔗糖的BUI，并逐渐下降。