Edwards J J, Tollaksen S L, Anderson N G
Clin Chem. 1982 Apr;28(4 Pt 2):941-8.
We mapped the proteins of human urine by high-resolution two-dimensional electrophoresis, utilizing the ISO-DALT system. Wide-range pH gradients and narrow-range acid gradients were both used in the first-dimension separations. The patterns revealed proteins ranging in relative molecular mass from 10 000 to 90 000. Proteins identified in the map included transferrin, albumin, hemopexin, alpha 2-HS glycoprotein, alpha 1-antitrypsin. Gc globulin, alpha 1-acid glycoprotein, Zn alpha 2-glycoprotein, retinol binding protein, beta 2-microglobulin, the immunoglobulin light chains, and MAUP (most acid urinary protein). The use and utility of internal-charge and molecular-mass standards are described. We used electrophoretic transfer of proteins to nitrocellulose sheets and subsequent detection by immunological methods to identify some proteins.
我们利用ISO-DALT系统，通过高分辨率二维电泳对人尿液中的蛋白质进行了图谱分析。在第一维分离中同时使用了宽范围pH梯度和窄范围酸性梯度。图谱显示相对分子质量范围为10000至90000的蛋白质。图谱中鉴定出的蛋白质包括转铁蛋白、白蛋白、血红素结合蛋白、α2-HS糖蛋白、α1-抗胰蛋白酶、Gc球蛋白、α1-酸性糖蛋白、锌α2-糖蛋白、视黄醇结合蛋白、β2-微球蛋白、免疫球蛋白轻链以及MAUP（最酸性尿蛋白）。描述了内部电荷和分子量标准的使用及效用。我们将蛋白质通过电泳转移至硝酸纤维素膜上，随后采用免疫方法进行检测以鉴定某些蛋白质。
