Basombrío M A, Besuschio S
Infect Immun. 1982 Apr;36(1):351-6. doi: 10.1128/iai.36.1.351-356.1982.
The development of chronic pathology in mice at 2 to 10 months after inoculation of 10(2) T. cruzi trypomastigotes can be prevented by preimmunization with live, attenuated culture parasites (strain TCC). Swiss mice received one or three immunizing inoculations of 10(6) TCC organisms and were challenged with 10(2) Tulahuén blood trypomastigotes. Control groups received only the immunizing or the challenge inoculations. Immunized groups as compared with nonimmunized controls had lower mortality rates at 2 months postchallenge (9% versus 23%; P = 0.059), lower early peaks of parasitemia, lower percentages of positive xenodiagnoses at 5.5 months (40 versus 80%; P = 0.061), and lower incidences of tissue lesions in the skeletal muscle (P less than 0.005) at 2,6, and 10 months postchallenge. Tissue lesions in the heart and smooth muscle were also reduced, reaching statistical significance after 10 months (P less than 0.02). Chronic pathology parameters were never enhanced in preimmunized groups. In spite of the putative role that autoimmunity may play in the development of chronic chagasic lesions, the preventive effect of vaccination is readily exerted upon the chronic murine model of Chagas' disease.
用活的减毒培养寄生虫（TCC株）进行预免疫可预防小鼠在接种10²个克氏锥虫锥鞭毛体后2至10个月出现慢性病理变化。瑞士小鼠接受10⁶个TCC生物体的一次或三次免疫接种，并用10²个图拉韦恩血液锥鞭毛体进行攻击。对照组仅接受免疫接种或攻击接种。与未免疫对照组相比，免疫组在攻击后2个月的死亡率较低（9%对23%；P = 0.059），寄生虫血症的早期峰值较低，在5.5个月时阳性异种诊断的百分比较低（40%对80%；P = 0.061），以及在攻击后2、6和10个月骨骼肌组织病变的发生率较低（P小于0.005）。心脏和平滑肌的组织病变也有所减少，在10个月后达到统计学意义（P小于0.02）。预免疫组的慢性病理参数从未增强。尽管自身免疫可能在慢性恰加斯病病变的发展中起作用，但疫苗接种的预防作用在恰加斯病慢性小鼠模型中很容易发挥。
