大鼠肝脏和肾脏中S-腺苷甲硫氨酸合成酶同工酶的免疫学区分
Immunological distinction of S-adenosylmethionine synthetase isozymes from rat liver and kidney.
作者信息
Abe T, Okada G, Teraoka H, Tsukada K
出版信息
J Biochem. 1982 Mar;91(3):1081-4. doi: 10.1093/oxfordjournals.jbchem.a133757.
The beta-form of S-adenosylmethionine synthetase among three isozymes has been purified from rat liver, and proven to be homogeneous. The molecular weight of this enzyme was estimated to be 100,000 by gel filtration on Sephacryl S-200, and the enzyme was shown to be composed of two subunits of 48,000 daltons. A rabbit antiserum against the normal rat liver beta-form of S-adenosylmethionine synthetase was used for immunochemical characterization. The alpha- and beta-forms of isozyme are immunochemically identical, but the antiserum did not react with the gamma-form from rat kidney.
已从大鼠肝脏中纯化出三种同工酶中的β型S-腺苷甲硫氨酸合成酶，并证明其为均一酶。通过在Sephacryl S-200上进行凝胶过滤，估计该酶的分子量为100,000，且该酶由两个48,000道尔顿的亚基组成。使用针对正常大鼠肝脏β型S-腺苷甲硫氨酸合成酶的兔抗血清进行免疫化学表征。同工酶的α型和β型在免疫化学上相同，但该抗血清与大鼠肾脏的γ型不发生反应。
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