Noguchi T, Fujiwara S
Biochem J. 1982 Jan 1;201(1):209-14. doi: 10.1042/bj2010209.
Glutamate:glyoxylate aminotransferase had been reported to be present exclusively in the peroxisomes of plant leaves and to participate in the glycollate pathway in leaf photorespiration (Tolbert (1971) Annu. Rev. Plant Physiol. 22, 45-74]. Glutamate:glyoxylate aminotransferase activity was already present in the etiolated cotyledons of cucumber (Cucumis sativus) seedlings, and increased during greening. The enzyme was present only in the cytosol of the etiolated cotyledons and appeared in the peroxisomes during greening. The enzyme was purified to homogeneity from the cytosol of the etiolated cotyledons and from the peroxisomes of the green cotyledons of cucumber seedlings. The two enzyme preparations had nearly identical enzymic and physical properties. On the basis of these findings, roles of glutamate:glyoxylate aminotransferase in the glycollate pathway in photorespiration, and the mechanism of its appearance in the peroxisomes during greening, are discussed.
乙醛酸转氨酶据报道仅存在于植物叶片的过氧化物酶体中，并参与叶片光呼吸中的乙醇酸途径[托尔伯特（1971年），《植物生理学年度评论》22卷，第45 - 74页]。谷氨酸：乙醛酸转氨酶活性在黄瓜（Cucumis sativus）幼苗的黄化子叶中就已存在，并在绿化过程中增加。该酶仅存在于黄化子叶的细胞质中，在绿化过程中出现在过氧化物酶体中。从黄瓜幼苗黄化子叶的细胞质和绿色子叶的过氧化物酶体中纯化得到了均一的该酶。这两种酶制剂具有几乎相同的酶学和物理性质。基于这些发现，讨论了谷氨酸：乙醛酸转氨酶在光呼吸乙醇酸途径中的作用及其在绿化过程中出现在过氧化物酶体中的机制。
