Løvø A, Hustvedt B E
Horm Metab Res. 1978 Jul;10(4):304-9. doi: 10.1055/s-0028-1093420.
The importance of the nutritional state for the development of metabolic alterations after ventromedial hypothalamic (VMH) destructions has been investigated. In the first experiment, groups of fed and 12 hours fasted animals were operated, given an intraperitoneal injection of 3H2O (1 mCi), and left without food until sacrificed 12 hours postoperatively. The lipogenesis in liver and adipose tissue, and the plasma levels of insulin, glucose, urea, free fatty acids (FFA) and triglycerides (TG) were measured. Animals operated in the fed state had increased lipogenesis in parametrial adipose tissue and hypoglycemia compared to their controls, whereas we found no differences between the groups which had been fasted 12 hours before surgery. In the second experiment, animals were deprived of food for 12 hours before being lesioned or sham-operated, and for 12 more hours postoperatively. They were then given one meal by stomach tube, and groups were killed 2, 4, 8 and 12 hours later. The following differences between groups were observed: 2 hours after feeding, the lesioned animals had increased plasma TG levels, but decreased liver lipogenesis. 4 hours after feeding, animals with lesions were hypoglycemic, and had lower plasma levels of FFA and TG than the controls. Hypoglycemia in lesioned animals was found also 8 hours after feeding, together with increased plasma TG levels. 12 hours after feeding, increased liver lipogenesis in lesioned animals was the only difference observed between the groups. The third and last experiment investigated the effect of three meals given 12, 18 and 24 hours postoperatively. Lesioned animals sacrificed 4 hours after the third meal had hypoglycemia, increased plasma TG levels, and increased lipogenesis, both in liver and adipose tissue. The metabolic differences between lesioned and sham-operated animals persisted also 8 hours after the last meal, except for the lipogenesis in adipose tissue. No differences in plasma insulin levels between animals with lesions or sham-operations were observed in these experiments.
研究了营养状态对腹内侧下丘脑（VMH）损毁后代谢改变发展的重要性。在第一个实验中，对进食组和禁食12小时的动物组进行手术，腹腔注射3H2O（1毫居里），术后12小时不给食物直至处死。测量肝脏和脂肪组织中的脂肪生成以及胰岛素、葡萄糖、尿素、游离脂肪酸（FFA）和甘油三酯（TG）的血浆水平。与对照组相比，在进食状态下进行手术的动物子宫旁脂肪组织中的脂肪生成增加且出现低血糖，而我们发现在手术前禁食12小时的各组之间没有差异。在第二个实验中，动物在损伤或假手术前禁食12小时，并在术后再禁食12小时。然后通过胃管给它们喂一餐，在2、4、8和12小时后处死各组。观察到各组之间有以下差异：喂食后2小时，损伤动物的血浆TG水平升高，但肝脏脂肪生成减少。喂食后4小时，有损伤的动物出现低血糖，且血浆FFA和TG水平低于对照组。喂食后8小时也发现损伤动物有低血糖，同时血浆TG水平升高。喂食后12小时，损伤动物肝脏脂肪生成增加是各组之间观察到的唯一差异。第三个也是最后一个实验研究了术后12、18和24小时给予三餐的影响。在第三餐4小时后处死的损伤动物出现低血糖、血浆TG水平升高以及肝脏和脂肪组织中的脂肪生成增加。除脂肪组织中的脂肪生成外，在最后一餐8小时后，损伤动物和假手术动物之间的代谢差异仍然存在。在这些实验中，未观察到损伤动物和假手术动物之间血浆胰岛素水平的差异。
