Pillai G K, Axelson J E, McErlane K M
J Chromatogr. 1982 Apr 16;229(1):103-9. doi: 10.1016/s0378-4347(00)86041-7.
Gas-liquid chromatography using capillary columns and electron-capture detection has been employed for the determination of a new antiarrhythmic drug, tocainide, in rat plasma and urine. The drug is extracted from a basified solution along with an internal standard, monoethylglycine xylidide, and subsequently reacted with heptafluorobutyric anhydride. The 50 m x 0.2 mm fused silica capillary column was coated in the laboratory with Carbowax 20M. Linearity of detector response was established in the range of 50-1000 ng of tocainide hydrochloride per 100 microliter of plasma or urine. This represented 14-270 pg of the free base at the detector, using a split ratio of 1:25 and an injection volume of 2 microliter. The derivatization method and chromatographic assay are well suited for monitoring of plasma or urine samples. The applicability of the method is demonstrated by the analysis of rat plasma collected over a period of 7 h after intravenous administration of 20 mg/kg of tocainide hydrochloride.
采用毛细管柱和电子捕获检测的气相色谱法已用于测定大鼠血浆和尿液中的一种新型抗心律失常药物妥卡尼。该药物与内标物单乙基甘氨酸二甲苯酰胺一起从碱化溶液中萃取出来，随后与七氟丁酸酐反应。50 m×0.2 mm的熔融石英毛细管柱是在实验室用Carbowax 20M涂覆的。检测器响应的线性范围为每100微升血浆或尿液中含有50 - 1000 ng盐酸妥卡尼。在分流比为1:25且进样体积为2微升的情况下，这相当于在检测器处有14 - 270 pg的游离碱。该衍生化方法和色谱分析方法非常适合监测血浆或尿液样本。静脉注射20 mg/kg盐酸妥卡尼后7小时内收集的大鼠血浆分析证明了该方法的适用性。
