Hozumi N, Wu G, Murialdo H, Baumal R, Mosmann T, Winberry L, Marks A
J Immunol. 1982 Jul;129(1):260-6.
The synthesis of lambda light chains and the arrangement of the lambda-chain genes was examined in cells of the mouse myeloma MOPC 315, which is an alpha lambda 2 producer, and in several mutants derived from it. The mutants produce lambda 2 chains only (MOPC 315.26, MOPC 315.34, and MOPC 315.37) or fail to produce alpha and lambda 2 chains (MOPC 315.25 and MOPC 315.36). Messenger RNA from the lambda 2 chain-producing cells directed the synthesis of a lambda 2 chain precursor and a fragment of the lambda 1 chain (lambda 1 F) in a wheat embryo cellfree system, whereas mRNA from the cells that do not produce lambda 2 chains directed the synthesis of lambda 1 F only. DNA from the parental MOPC 315 cells and from the lambda 2 chain-producing cells contained discrete EcoRI restriction fragments coding for rearranged lambda 1 and lambda 23 chain genes and their respective germ-line V and J-C regions. DNA from the no-Ig-producing cells contained fragments coding for the rearranged lambda 1 chain gene and the germ-line V lambda 2 region, but it lacked the sequences coding for the rearranged lambda 2 chain gene and the germ-line V lambda 1 and J-C lambda 1 regions. These results suggest that rearrangements of the lambda 1 and lambda 2 chain genes occur on different chromosomes in MOPC 315 cells and imply that rearrangements of the lambda 1 and lambda 2 chain genes on the same chromosome may be mutually exclusive.
在小鼠骨髓瘤MOPC 315（一种αλ2产生细胞）及其衍生的几个突变体的细胞中，研究了λ轻链的合成及λ链基因的排列。这些突变体要么只产生λ2链（MOPC 315.26、MOPC 315.34和MOPC 315.37），要么不产生α链和λ2链（MOPC 315.25和MOPC 315.36）。来自产生λ2链细胞的信使RNA在小麦胚无细胞系统中指导合成λ2链前体和λ1链片段（λ1F），而来自不产生λ2链细胞的mRNA仅指导合成λ1F。亲本MOPC 315细胞和产生λ2链细胞的DNA含有离散的EcoRI限制片段，编码重排的λ1和λ23链基因及其各自的种系V和J-C区域。不产生免疫球蛋白细胞的DNA含有编码重排的λ1链基因和种系Vλ2区域的片段，但缺乏编码重排的λ2链基因以及种系Vλ1和J-Cλ1区域的序列。这些结果表明，在MOPC 315细胞中，λ1和λ2链基因的重排在不同染色体上发生，这意味着同一染色体上λ1和λ2链基因的重排可能相互排斥。
