异源嵌合体小鼠对合成聚合物GL phi的免疫反应。III. 抗GL phi抗体的同种异型分析。

The immune response of allophenic mice to the synthetic polymer GL phi. III. Allotype analysis of anti-GL phi antibody.

作者信息

Gorcyca D E, McIvor-Nayeri J L, Maurer P H, Warner C M

出版信息

J Immunogenet. 1982 Apr;9(2):83-92. doi: 10.1111/j.1744-313x.1982.tb00967.x.

DOI:10.1111/j.1744-313x.1982.tb00967.x

Abstract

Allophenic mice produced by aggregating 8-cell embryos from a low responder (CBA) and a high responder (DBA/1) strain to the synthetic amino acid polymer GL phi.. In some strains of mice the immune response to this polymer is controlled by two genes, alpha and beta, which map to the IE/C and IA subregions of the H-2 complex, respectively. The CBA strain is alpha+beta--whereas the DBA/1 strain can be considered to be alpha+beta+. These two strains also differ in immunoglobulin allotype: CBA is allotype alpha and DBA/1 is allotype c. Eight CBA--DBA/1 allophenic mice were immunized with GL phi.. Four of the mice gave a high secondary response to the polymer. The allotype of the antibody from these mice was determined by affinity chromatography, and it was found that only allotype c was present. This means that all of the antibody was produced by high responder DBA/1 cells. Thus, there is no evidence for the cooperation of histoincompatible cells in the mounting of an immune response by allophenic mice.

摘要

通过将低反应性（CBA）和高反应性（DBA/1）品系的8细胞胚胎与合成氨基酸聚合物GL phi聚集产生的异表型小鼠。在某些小鼠品系中，对这种聚合物的免疫反应由两个基因α和β控制，它们分别定位于H-2复合体的IE/C和IA亚区。CBA品系是α+β-，而DBA/1品系可被认为是α+β+。这两个品系在免疫球蛋白同种异型上也有所不同：CBA是同种异型α，DBA/1是同种异型c。八只CBA-DBA/1异表型小鼠用GL phi免疫。其中四只小鼠对该聚合物产生了强烈的二次反应。通过亲和层析确定了这些小鼠抗体的同种异型，发现只存在同种异型c。这意味着所有抗体都是由高反应性的DBA/1细胞产生的。因此，没有证据表明异表型小鼠在引发免疫反应时组织不相容细胞之间存在合作。