Hall C L, Hamilton P B
Poult Sci. 1982 Jan;61(1):62-6. doi: 10.3382/ps.0610062.
Gentian violet, a compound that gained wide acceptance in the poultry industry as a mold inhibitor in feed, was tested for its activity against eight aflatoxigenic strains of Aspergillus flavus and A. Parasiticus. In a simple nutrient medium, it completely inhibited growth of all strains at 8 micrograms/ml. When chicken feed was added to the medium, the inhibition by the same concentration ranged from 12 to 44% depending on the strain. Observations suggested that both the feed and the fungi were involved in the resistance conferred by chicken feed. Growth and aflatoxin production on moist rice was inhibited in one strain but not in a second strain. Inoculating autoclaved chicken feed of varying moisture contents with pure cultures revealed that the activity of gentian violet was better at high moisture contents than at low moisture contents approximating those found in poultry feed. It was not possible to assess the activity of gentian violet in unaltered chicken feed. Attempts failed because the formation of reproducible levels of aflatoxin could not be attained.
龙胆紫是一种在禽类养殖业中作为饲料霉菌抑制剂而被广泛接受的化合物，已对其针对八株产黄曲霉毒素的黄曲霉和寄生曲霉菌株的活性进行了测试。在一种简单的营养培养基中，它在8微克/毫升时能完全抑制所有菌株的生长。当向培养基中添加鸡饲料时，相同浓度的抑制率因菌株而异，范围在12%至44%之间。观察结果表明，饲料和真菌都与鸡饲料所赋予的抗性有关。在一个菌株中，潮湿大米上的生长和黄曲霉毒素产生受到抑制，但在另一个菌株中则未受抑制。用纯培养物接种不同水分含量的高压灭菌鸡饲料表明，龙胆紫在高水分含量时的活性比在接近禽类饲料中发现的低水分含量时更好。无法评估龙胆紫在未改变的鸡饲料中的活性。尝试失败了，因为无法达到可重复的黄曲霉毒素水平。
