Banerjee A
Biochem Pharmacol. 1982 Apr 1;31(7):1333-6. doi: 10.1016/0006-2952(82)90025-9.
The effects of sodium valproate and acetazolamide on the oxygen uptake of guinea pig brain cortex slices were investigated. In calcium-free medium, sodium valproate inhibited the oxygen uptake appreciably in the presence of glucose and glutamic acid. Acetazolamide, on the other hand, was more effective in inhibiting oxygen uptake in the presence of glucose than in the presence of glutamic acid. Addition of 0.2 mM CaCl2 in the medium containing 5 mM KCl could appreciably reverse the inhibition of oxygen uptake by acetazolamide in the presence of glucose. The inhibition of oxygen uptake by these drugs in the presence of glucose, however, could be completely reversed by increasing the dose of K+ ions (100 mM) in the medium which had no effect on the inhibition of oxygen uptake in the presence of glutamic acid. When the concentration of Ca2+ ions in the medium was elevated to 0.75 mM, the inhibitory effects of these drugs on the oxygen uptake in the presence of both glucose and glutamic acid could be completely abolished. Sodium valproate also inhibited the endogenous respiration of guinea pig brain cortex slices, whereas acetazolamide was almost without any effect. Increase in the concentration of Ca2+ ions in the medium failed to counteract the inhibition of endogenous respiration of guinea pig brain cortex slices by sodium valproate.
研究了丙戊酸钠和乙酰唑胺对豚鼠脑皮质切片氧摄取的影响。在无钙培养基中，丙戊酸钠在有葡萄糖和谷氨酸存在的情况下显著抑制氧摄取。另一方面，乙酰唑胺在有葡萄糖存在时比在有谷氨酸存在时更有效地抑制氧摄取。在含有5 mM氯化钾的培养基中添加0.2 mM氯化钙可明显逆转乙酰唑胺在有葡萄糖存在时对氧摄取的抑制作用。然而，通过增加培养基中钾离子（100 mM）的剂量可完全逆转这些药物在有葡萄糖存在时对氧摄取的抑制作用，而这对在有谷氨酸存在时氧摄取的抑制没有影响。当培养基中钙离子浓度升高到0.75 mM时，这些药物在有葡萄糖和谷氨酸存在时对氧摄取的抑制作用可完全消除。丙戊酸钠也抑制豚鼠脑皮质切片的内源性呼吸，而乙酰唑胺几乎没有任何作用。培养基中钙离子浓度的增加未能抵消丙戊酸钠对豚鼠脑皮质切片内源性呼吸的抑制作用。
