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抗洋地黄毒苷单克隆抗体的制备(作者译)

[Production of an anti-digitalin monoclonal antibody (author's transl)].

作者信息

Edelman L, Colignon A, Scherrmann J M, Fournier E, Reviron J, Bach J F

出版信息

C R Seances Acad Sci III. 1982 Mar 8;294(10):421-4.

PMID:6807500
Abstract

Anti-digitalin monoclonal antibodies were obtained by hybridization of lymphoid cells from mice immunized with the drug and the 8653 myeloma cells from BALB/c Mice. Two of these antibodies were submitted to immunochemical analysis which revealed that they belonged to IgM and IgGl classes, and the high affinity of one of them (1,2 x 10(9) M(-1)). The availability os such antibodies opens new perspectives for the treatment of digitalin intoxication and the possibility of establishing a standardized radioimmunoassay.

摘要

通过用洋地黄毒苷免疫的小鼠的淋巴细胞与来自BALB/c小鼠的8653骨髓瘤细胞杂交,获得了抗洋地黄毒苷单克隆抗体。对其中两种抗体进行了免疫化学分析,结果表明它们属于IgM和IgG1类,并且其中一种抗体具有高亲和力(1.2×10⁹ M⁻¹)。这类抗体的可用性为洋地黄毒苷中毒的治疗开辟了新的前景,并为建立标准化放射免疫测定提供了可能性。

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