Verma D S, Sunkara P S
Cancer Res. 1982 Aug;42(8):3046-9.
The role of polyamines during the process of colony-stimulating factor-induced proliferation and differentiation of human granulocyte-macrophage progenitor cells was studied using in vitro liquid cultures and semisolid agar cultures. Human placenta-conditioned medium, a partially purified preparation of colony-stimulating factor, rapidly and significantly increased the intracellular putrescine concentration of light-density, non-adherent, T-lymphocyte-depleted human marrow cells, Inhibition of polyamine biosynthesis with alpha-difluoromethylornithine, a catalytic, irreversible inhibitor or ornithine decarboxylase, resulted in an accumulation of blasts, promyelocytes, and myelocytes with a concomitant decrease in metamyelocytes and polymorphonuclear granulocytes, suggesting a granulopoietic differentiation block. The observed inhibition of differentiation could easily be reversed with exogenously added putrescine. These data indicate clearly an essential role for polyamines during the process of human granulopoietic differentiation.
利用体外液体培养和半固体琼脂培养技术，研究了多胺在集落刺激因子诱导人粒细胞-巨噬细胞祖细胞增殖和分化过程中的作用。人胎盘条件培养基是一种部分纯化的集落刺激因子制剂，可迅速且显著提高低密度、非贴壁、T淋巴细胞去除的人骨髓细胞的细胞内腐胺浓度。用α-二氟甲基鸟氨酸（一种催化性、不可逆的鸟氨酸脱羧酶抑制剂）抑制多胺生物合成，导致原始细胞、早幼粒细胞和中幼粒细胞积累，同时晚幼粒细胞和多形核粒细胞减少，提示粒细胞生成分化受阻。观察到的分化抑制可通过外源添加腐胺轻易逆转。这些数据清楚地表明多胺在人类粒细胞生成分化过程中起着重要作用。
