Seidler E
Acta Histochem. 1982;70(1):106-14.
During the incubation of cryostat sections (various organs of rat) with aqueous media xanthine dehydrogenase and superoxide dismutase activities become soluble and detectable in the solution by biochemical methods. Using the tetrazolium technique the demonstration of xanthine dehydrogenase is successful only after separation from the superoxide dismutase by gel electrophoresis. The soluble flavine enzymee xanthine dehydrogenase is taken as an example to discuss the significance of superoxide dismutases in incubation media for the histochemical demonstration of dehydrogenating enzymes by tetrazolium methods.
在对低温恒温器切片（大鼠的各种器官）与水性介质进行孵育期间，黄嘌呤脱氢酶和超氧化物歧化酶的活性会溶解，并可通过生化方法在溶液中检测到。使用四唑盐技术，只有在通过凝胶电泳从超氧化物歧化酶中分离出来后，才能成功显示黄嘌呤脱氢酶。以可溶性黄素酶黄嘌呤脱氢酶为例，讨论超氧化物歧化酶在孵育介质中对用四唑盐方法进行脱氢酶组织化学显示的意义。
