胺类物质对未转化的3T3细胞被伴刀豆球蛋白A凝集的敏感性以及长春碱诱导副晶形成的影响。
Effects of amine substances on susceptibility of cells to agglutination by concanavalin A and on paracrystal formation by vinblastine in untransformed 3T3 cells.
作者信息
Saito K, Kumagai K
出版信息
Tohoku J Exp Med. 1982 May;137(1):91-9. doi: 10.1620/tjem.137.91.
The effects of a secondary amine (ketamine), tertiary amines (dibucaine, lidocaine, marcaine, propanidid, diazepam and chlorpromazine) and a quaternary amine (tetraethylammonium bromide, TEA) on mouse 3T3 cell agglutination by concanavalin A (Con A), on patch formation of Con A receptors on the cell surface, and on paracrystal formation by vinblastine in cytoplasm were studied. These amines enhanced the cell agglutination at low concentrations of Con A, as did the mixture of colchicine and cytochalasin B. Ca++, applied extracellularly, inhibited the effects of these amines on cell agglutination by Con A. The patch formation of Con A receptors on the cell surface as revealed by fluoresceinated Con A was enhanced by these amines. Ketamine, dibucaine and TEA inhibited the paracrystal formation in cytoplasm, as did Ca++ ionophores such as A-23187 and X-537-A. These results suggest that the amines tested affect the fluidity of Con A receptors by impairment of cell membrane structural proteins and result in the increase of the susceptibility of cells to agglutination by Con A.
研究了仲胺（氯胺酮）、叔胺（丁卡因、利多卡因、布比卡因、丙泮尼地、地西泮和氯丙嗪）和季胺（溴化四乙铵，TEA）对伴刀豆球蛋白A（Con A）诱导的小鼠3T3细胞凝集、细胞表面Con A受体斑片形成以及长春花碱在细胞质中形成副晶体的影响。这些胺类在低浓度Con A时增强细胞凝集，秋水仙碱和细胞松弛素B的混合物也有此作用。细胞外施加的Ca++抑制了这些胺类对Con A诱导的细胞凝集的作用。荧光标记的Con A显示，这些胺类增强了细胞表面Con A受体的斑片形成。氯胺酮、丁卡因和TEA抑制细胞质中的副晶体形成，A-23187和X-537-A等Ca++离子载体也有此作用。这些结果表明，所测试的胺类通过损害细胞膜结构蛋白影响Con A受体的流动性，导致细胞对Con A凝集的敏感性增加。
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