Gibbons S, Kelton J G
Transfusion. 1982 Jul-Aug;22(4):295-7. doi: 10.1046/j.1537-2995.1982.22482251211.x.
The complexity of assays used to measure platelet-associated IgG (PAIgG) often requires blood samples to be sent to a central laboratory, In order to determine the optimal storage conditions, we measured PAIgG, platelet recovery, and platelet size serially over several days in whole blood samples obtained from healthy individuals. Blood was collected into EDTA, acid-citrate-dextrose (ACD) and citrate-phosphate-dextrose-adenine (CPD-A) with and without the fixative paraformaldehyde (P). The level of PAIgG remained with normal limits in all samples stored for up to six days in the ACD, the CPD-A and the CPD-A-P samples. The least drop in platelet count and change in platelet size occurred in the ACD and CPD-A samples. Blood collected into EDTA plus paraformaldehyde was less satisfactory, with PAIgG rising by day three in some samples, and a decline in the platelet count occurring by day two. Specimens collected into EDTA alone were least satisfactory, with a rise in PAIgG by 24 hours after collection. Collection of whole blood into either ACD or CPD-A and storage at 22 degrees C allows a good recovery of platelets whose level of PAIgG is unchanged for up to six days.
用于测量血小板相关免疫球蛋白（PAIgG）的检测方法复杂，通常需要将血样送到中心实验室。为了确定最佳储存条件，我们对从健康个体采集的全血样本在数天内连续测量了PAIgG、血小板回收率和血小板大小。血液被采集到含有和不含有固定剂多聚甲醛（P）的乙二胺四乙酸（EDTA）、枸橼酸-葡萄糖（ACD）和枸橼酸-磷酸盐-葡萄糖-腺嘌呤（CPD-A）中。在ACD、CPD-A和CPD-A-P样本中，所有储存长达6天的样本中PAIgG水平均保持在正常范围内。血小板计数下降最少且血小板大小变化最小的是ACD和CPD-A样本。采集到EDTA加甲醛中的血液不太理想，一些样本中PAIgG在第3天升高，血小板计数在第2天下降。单独采集到EDTA中的样本最不理想，采集后24小时内PAIgG就升高。将全血采集到ACD或CPD-A中并在22℃储存，可使血小板良好回收，其PAIgG水平在长达6天内保持不变。
