糖胺聚糖对分离的正常和癌细胞核内源性DNA合成的影响。肝素的差异作用。

Influence of glycosaminoglycans on endogenous DNA synthesis in isolated normal and cancer cell nuclei. Differential effect of heparin.

作者信息

Furukawa K, Bhavanandan V P

出版信息

Biochim Biophys Acta. 1982 Jun 30;697(3):344-52. doi: 10.1016/0167-4781(82)90098-7.

Abstract

The influence of exogenously-added glycosaminoglycans and glycoproteins on DNA synthesis in isolated nuclei, from normal and malignant tissues, was investigated. Heparin stimulated DNA synthesis in normal cell nuclei at concentrations (heparin/DNA (w/w) less than 0.9) which inhibited DNA synthesis in tumor cell nuclei. At higher concentrations (heparin/DNA (w/w greater than 0.9) heparin inhibited DNA synthesis in both normal and tumor cell nuclei. The chondroitin-4 and 6-sulfates, heparin sulfate, cartilage proteoglycan, N-desulfated heparin, and glycophorin caused inhibition of DNA synthesis at all concentrations tested and in all nuclei examined. Hyaluronic acid, dermatan sulfate, keratan sulfate, alpha 1-acid glycoprotein and fetuin had no significant influence on DNA synthesis in isolated nuclei.

摘要

研究了外源性添加的糖胺聚糖和糖蛋白对正常组织和恶性组织分离细胞核中DNA合成的影响。肝素在浓度（肝素/DNA（w/w）小于0.9）时刺激正常细胞核中的DNA合成，而该浓度会抑制肿瘤细胞核中的DNA合成。在更高浓度（肝素/DNA（w/w）大于0.9）时，肝素抑制正常细胞核和肿瘤细胞核中的DNA合成。硫酸软骨素-4和-6、硫酸乙酰肝素、软骨蛋白聚糖、N-去硫酸化肝素和血型糖蛋白在所有测试浓度下和所有检测的细胞核中均导致DNA合成受到抑制。透明质酸、硫酸皮肤素、硫酸角质素、α1-酸性糖蛋白和胎球蛋白对分离细胞核中的DNA合成没有显著影响。