Alam T, Finkelstein D, Srere P A
J Biol Chem. 1982 Sep 25;257(18):11181-5.
The citrate synthase of yeast was purified to homogeneity and shown to have a subunit molecular weight of 52,000. Antibodies were prepared to it in rabbits. Translation of total yeast mRNA using a reticulocyte lysate showed that [3H]leucine was incorporated into a protein precipitable by rabbit anti-yeast citrate synthase with a molecular weight of about 54,000. No incorporation of [35S]methionine into an anti-citrate synthase precipitable protein could be detected in a similar experiment. In vivo labeling of cells with 35SO4 did not result in the labeling of citrate synthase.
酵母柠檬酸合酶被纯化至同质，其亚基分子量为52,000。用兔子制备了针对它的抗体。使用网织红细胞裂解物对酵母总mRNA进行翻译，结果显示[3H]亮氨酸被掺入一种分子量约为54,000、可被兔抗酵母柠檬酸合酶沉淀的蛋白质中。在类似实验中，未检测到[35S]甲硫氨酸掺入抗柠檬酸合酶可沉淀的蛋白质中。用35SO4对细胞进行体内标记未导致柠檬酸合酶被标记。
