一种用于高压停流装置的观察室。

An observation chamber for a high-pressure stopped-flow apparatus.

作者信息

Smith P D, Beile E E, Berger R L

出版信息

J Biochem Biophys Methods. 1982 Jun;6(2):173-7. doi: 10.1016/0165-022x(82)90063-x.

Abstract

A new design of the observation window to observation chamber seal is described for the Berger rapid stopped-flow apparatus. This design permits reliable operation of the apparatus and retains the features of a square non-deviating observation path with flush windows. Incorporation of fiber optic light guides for the observation path has made the apparatus insensitive to vibration artifacts. A demonstration reaction of calcium binding to EGTA is presented; the reaction, which is 90% complete within 2 ms, illustrates the capability of this apparatus for examining fast reactions.

摘要

本文描述了一种用于伯杰快速停流装置的观察窗与观察室密封的新设计。这种设计使该装置能够可靠运行，并保留了方形无偏差观察路径和平齐窗口的特点。观察路径采用光纤光导，使该装置对振动伪影不敏感。文中给出了钙与乙二醇双四乙酸（EGTA）结合的演示反应；该反应在2毫秒内完成90%，说明了该装置用于检测快速反应的能力。