Iozzo R V, MacDonald G H, Wight T N
J Histochem Cytochem. 1982 Jul;30(7):697-701. doi: 10.1177/30.7.6809811.
The immunologic localization of catalase in human eosinophilic leukocytes was studied by use of rabbit anti-human erythrocyte catalase (aHEC) antiserum. Indirect immunofluorescence studies revealed intense localization of fluorescence in the cytoplasm of the eosinophils with a pattern of distribution corresponding to that of the eosinophil granules. Control samples and other nucleated blood elements remained essentially unreactive. Immunoelectron microscopy using aHEC coupled to protein A-gold complexes revealed that catalase was present exclusively within the cytoplasmic granules and equally distributed over the paracrystalline body and the homogeneous granule matrix. The presence of catalase within the paracrystalline body has not been demonstrated before with conventional cytochemical techniques. The results raise the possibility that catalase is stored in the paracrystalline body in an inactive or latent form readily available during eosinophil activation and granule release.
利用兔抗人红细胞过氧化氢酶（aHEC）抗血清研究了过氧化氢酶在人嗜酸性粒细胞中的免疫定位。间接免疫荧光研究显示，嗜酸性粒细胞胞质内有强烈的荧光定位，其分布模式与嗜酸性粒细胞颗粒的分布模式一致。对照样本和其他有核血细胞基本无反应。使用与蛋白A-金复合物偶联的aHEC进行免疫电子显微镜研究显示，过氧化氢酶仅存在于胞质颗粒内，并均匀分布于类晶体和均匀的颗粒基质上。用传统细胞化学技术以前未证明类晶体内存在过氧化氢酶。这些结果增加了一种可能性，即过氧化氢酶以无活性或潜伏形式储存在类晶体内，在嗜酸性粒细胞激活和颗粒释放过程中易于利用。
