Owens N A, Hui H L, Green F A
J Immunol. 1982 Oct;129(4):1471-3.
The relation of the human erythrocyte Rho(D) antigen to its chimpanzee counterpart is currently unresolved. To explore this relation, we prepared 14C protein A to compare the binding parameters between human anti-Rho(D) and Rho(D)-positive human and chimpanzee erythrocytes. Double reciprocal plots of the binding in each case exhibited a straight line with intercepts indicating approximately the same numbers of Rho(D) binding sites for human and chimpanzee erythrocytes but an affinity that was 20 times greater for the human. This would indicate that the human and chimpanzee each have a single class of sites whose primary structure is very similar but sufficiently different to give the large divergence in affinity as measured by the human isoimmune antiserum.
人类红细胞Rho(D)抗原与其黑猩猩对应物之间的关系目前尚未明确。为了探究这种关系，我们制备了14C蛋白A，以比较人抗Rho(D)与Rho(D)阳性的人类和黑猩猩红细胞之间的结合参数。每种情况下结合的双倒数图均呈现为一条直线，截距表明人类和黑猩猩红细胞上的Rho(D)结合位点数量大致相同，但人类的亲和力比黑猩猩大20倍。这表明人类和黑猩猩各自都有一类位点，其一级结构非常相似，但又存在足够差异，从而导致人同种免疫抗血清测得的亲和力有很大差异。
