Donath K, Breuner G
J Oral Pathol. 1982 Aug;11(4):318-26. doi: 10.1111/j.1600-0714.1982.tb00172.x.
A new sawing-grinding method is described for the histological evaluation of jaw bones with teeth or bones containing implants (ceramic or metallic). The undecalcified bone is embedded in acrylic resin and sawed at 100 to 150 micrometers. The slices are ground automatically by a special machine to a thickness of 5-10 micrometers. The usually employed staining procedures for hard plastic embedded-tissues may be used. Plaque, fillings, crowns, bridges, implants and soft tissues are preserved in situ. Macroscopic and microscopic detail of good quality is preserved for histological and morphometrical evaluation.
本文描述了一种新的锯磨方法,用于对带有牙齿的颌骨或含有植入物(陶瓷或金属)的骨骼进行组织学评估。未脱钙的骨块被嵌入丙烯酸树脂中,并以100至150微米的厚度进行锯切。切片由一台特殊机器自动研磨至5 - 10微米的厚度。对于硬塑料包埋组织,可采用通常使用的染色程序。菌斑、填充物、牙冠、牙桥、植入物和软组织均原位保存。高质量的宏观和微观细节得以保留,用于组织学和形态计量学评估。