Ichikawa Y, Hiwatashi A
Biochim Biophys Acta. 1982 Jul 12;705(1):82-91. doi: 10.1016/0167-4838(82)90339-9.
Cytochrome P-450scc from bovine adrenocortical mitochondria was found to be a glycoprotein, similar to NADPH-adrenodoxin reductase. The role of sugar moieties of components of the cytochrome P-450-linked mixed-function oxidase system was investigated. The NADPH-ferricyanide reductase activity of NADPH-adrenodoxin reductase was not affected by neuraminidase treatment, but NADPH-cytochrome c reductase activity of NADPH-adrenodoxin reductase and adrenodoxin was lost. Cytochrome P-450scc treated with neuraminidase could not be reduced by NADPH-adrenodoxin reductase and adrenodoxin, and the side-chain cleavage activity of cholesterol by the cytochrome P-450-linked mixed-function oxidase system was lost by neuraminidase treatment of cytochrome P-450scc or NADPH-adrenodoxin reductase or both. These results indicate that the sugar moieties of the components of the cytochrome P-450-linked mixed-function oxidase system are necessary for binding and attaching of the components and electron transport.
已发现牛肾上腺皮质线粒体中的细胞色素P-450scc是一种糖蛋白，类似于NADPH-肾上腺皮质铁氧还蛋白还原酶。对细胞色素P-450相关混合功能氧化酶系统各组分糖基部分的作用进行了研究。NADPH-肾上腺皮质铁氧还蛋白还原酶的NADPH-铁氰化物还原酶活性不受神经氨酸酶处理的影响，但NADPH-肾上腺皮质铁氧还蛋白还原酶和肾上腺皮质铁氧还蛋白的NADPH-细胞色素c还原酶活性丧失。经神经氨酸酶处理的细胞色素P-450scc不能被NADPH-肾上腺皮质铁氧还蛋白还原酶和肾上腺皮质铁氧还蛋白还原，并且通过细胞色素P-450相关混合功能氧化酶系统对胆固醇的侧链裂解活性因对细胞色素P-450scc或NADPH-肾上腺皮质铁氧还蛋白还原酶或两者进行神经氨酸酶处理而丧失。这些结果表明，细胞色素P-450相关混合功能氧化酶系统各组分的糖基部分对于各组分的结合与附着以及电子传递是必需的。
